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Application Scenarios
R & D, production, sales and service of Life science instruments
Fluorometer Application
The fluorometer combined with the quantitative kit, using fluorescent dyes to selectively bind to specific target molecules. It is more accurate than the traditional UV absorption method since the UV absorption method is not selective. It measures the absorption value of all substances at 260nm - such as DNA, RNA, proteins, degraded nucleic acids and free nucleotides or excess salt ions. This product is a highly sensitive fluorometer specially designed to detect concentrations in a lower limit than microvolume UV-Vis spectrophotometer.
Application Direction
  • Quantification of specific biomolecular
  • Quantification of low concentration nucleic acid/protein
  • Sample impurity screening and error elimination
  • Routine nucleic acid/protein quantification
  • Quantification of specific biomolecular
    Fluorescence quantitative detection methods use fluorescent dyes to selectively bind to specific target molecules. It’s more accurate than traditional UV absorption methods, because UV absorption methods are not selective and will measure the absorption value of all substances at the selected wavelength - such as DNA, RNA, proteins, degraded nucleic acids and free nucleotides or excess salt ions.
  • Quantification of low concentration nucleic acid/protein
    This product is a highly sensitive fluorometer specially designed to detect concentrations in a lower limit than a microvolume UV-Vis spectrophotometer. Because the fluorescence dye binding reaction will exponentially amplify the concentration value of the sample to be measured, it is suitable for the detection of microvolume or even trace biomolecules.
  • Sample impurity screening and error elimination
    Since the fluorometer can specifically detect the exact concentration of the target molecule in the sample, and the ultraviolet spectrophotometry can detect the total absorbance of the sample at the selected wavelength, the combination of the two results can determine the concentration and type of impurities in the sample.
  • Routine nucleic acid/protein quantification
    Due to the fixed detection process, fluorescence quantitative experiment is convenient for batch operation, and is suitable for high-throughput routine quantitative detection of different samples of the same substance.
Application Area
  • Molecular biology
    Fluorometer is generally suitable for all biomolecules with corresponding fluorescent dyes
  • Cell biology
    The specific binding of fluorescent dyes to target molecules provides a good basis for cell metabolic kinetics analysis
Detection Process
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