杭州优米仪器有限公司-应用场景

Micro-spectrophotometer Application

Micro-spectrophotometer is a highly reproducible full-wavelength spectrophotometer that uses both pedestal and cuvette detection modes. Rapid and accurate detection of nucleic acids, proteins and cellular solutions. The sample detection concentration range is large and the operation is simple. It not only can be used to measure DNA, RNA purity and concentration, but also can be used for general substance analysis absorbance detection.

Application Direction

Quantitative analysis of DNA and RNA
Quantitative analysis of protein
OD600 bacterial solution detection
UV-Vis

Quantitative analysis of DNA and RNA

The micro-spectrophotometer calculates the concentration of nucleic acid in the sample by measuring the light absorption value (OD value) at 260nm, and calculates the ratio of absorbance at 260nm and 280nm to determine the content of protein/hydrolyzed salt impurities in the sample.
Quantitative analysis of protein

The micro-spectrophotometer calculates the concentration of protein (or hydrolyzed polypeptide molecules) in the sample by measuring the value of light absorption (OD value) at 280nm (or 205nm). The instrument has built-in standard curves for globulins, antibody proteins, and enzymes.
OD600 bacterial solution detection

The OD600 mode uses a 1cm glass cuvette with a conventional capacity of 1.5-4mL.
UV-Vis

The optical range of this instrument is 190-850nm, 1nm step, UV-Vis mode can be used for spectral scanning of samples.

Application Area

Molecular biology

Nucleic acid/protein quantification
Microbiology

Qualitative of nucleic acid and quantification of antibody protein
Animal Physiology

Quantitative and qualitative detection of enzyme protein molecules
Cell signal transduction and drug screening

Cell signal transduction protein real-time monitoring and analysis of specific activity of enzymes